TY - JOUR AU - Kutsenko, O. K. AU - Gorbenko, G. P. AU - Trusova, V. M. PY - 1970/01/01 Y2 - 2024/03/28 TI - Hemoglobin binding to phospholipid membranes as revealed by pyrene fluorescence study JF - Biophysical Bulletin JA - Bioph. Bull. VL - 1 IS - 26 SE - Cell biophysics DO - UR - https://periodicals.karazin.ua/biophysvisnyk/article/view/2710 SP - AB - In this work hemoglobin (Hb) association with lipid bilayers was investigated using fluorescent probepyrene. Model membranes were prepared from zwitterionic lipid phosphatidylcholine (PC), anionic lipidphosphatidylglycerol (PG) and cholesterol (Chol). Hb-lipid binding was followed by the pyrenefluorescence quenching. Hb-induced decrease of pyrene monomer fluorescence was followed by theincrease of relative intensities of vibronic bands. Presumably, Hb penetration into the bilayer increasesthe space between neighbouring lipids and promotes water penetration into the membrane core. Pyreneexcimer emission quenching was interpreted in terms of resonance energy transfer. The greatest depth ofHb penetration into the lipid bilayer was observed in PC vesicles. In Chol-containing liposomes sterolcondensing effect prevents deep protein penetration into the membrane. PG has an ability to stabilize lipidbilayers due to the ordered state of its lipid tails and H-bonding interactions between lipid molecules. Thisalso can prevent Hb access to the inner membrane regions. ER -