TY - JOUR AU - Shckorbatov, Yu. G. AU - Berezhnoy, A. Yu. PY - 2006/06/06 Y2 - 2024/03/28 TI - The DNA chains interaction energy in E. coli promoters in connection with their functional activity JF - Biophysical Bulletin JA - Bioph. Bull. VL - 1 IS - 17 SE - Molecular biophysics DO - UR - https://periodicals.karazin.ua/biophysvisnyk/article/view/12848 SP - 42-47 AB - The calculations of interaction energy of complementary nucleotides in promoter were performed. The methods of graphic representation of energetic promoter properties were elaborated. The data obtained indicate that the energy distribution through the promoter length has 3 minimums centered at the positions -35, -8 and +7 nucleotide number from the starting site of transcription. The most important sequence (Pribnow box) differs from the rest of promoter in its low energy of interaction between DNA chains. We defined 4 clusters in Pribnow box and in -35 sequences. The negative correlation between the mean DNA chains interaction energy in the clusters in Pribnow box and promoter strength was determined. Quite on the contrary in -35 box the mean DNA chains interaction energy directly correlate with promoter strength. ER -