Agrobacterium-mediated in planta transformation of bread winter wheat cv. Podolianka
Abstract
Recently the method of plant genetic transformation in planta involving Agrobacterium has increasingly become popular. It turned to be more competitive in relation to the “classical” methods – Agrobacterium-mediated in vitro transformation and biolistic transformation. The possibility to obtain genetic transformants of winter wheat Triticum aestivum cv. Podolianka by Agrobacterium-mediated in planta transformation is proved in this article. In the study we used ABI strain of Agrobacterium tumefaciens which contained the binary vector p014 with coding sequences of nptII and sgfp genes. The bacterial suspension was applied to 24 pre-neutered wheat spikes. After pollination 293 grains were collected. Analysis of T0 plants for the presence of transgenes was performed by PCR. Among the 175 analyzed plants 9 showed the presence of nptII gene and 4 of them had sequences of both nptII and sgfp genes.
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