Dynamics of hematopoiesis regulators content in bone marrow under the poultry embryonic tissue extract usage for the radiation-caused immune damage correction

Abstract

The aim of our study was to examine the effect of the poultry embryonic tissue extract (PETE) application at G-CSF, Flt3-ligand and IL-6 levels in the bone marrow cells supernatant of mice with radiation-caused immune disease. The PETE usage led to G-CSF levels increasing between 1st and 3rd day of observation, on the 7th day its level was at the normal rate. The comparative drug applying led to some stimulation of G-CSF levels, but compared to the PETE usage not so prolonged. During the early hours after depressive exposure under PETE treatment there was a significant IL-6 synthesis stimulation. At comparative drug applying its significant increase was registered only in the period from 16th to 24th hour. Influenced by PETE usage the Flt3-ligand level in mice with radiation-induced immune damage did not show such increase, which was observed in control animals – since the 3rd day their values were normal. At comparative drug applying the Flt3-ligand value did not reach normal rate on the 21st day of observation. Thus, due to the stimulation of the synthesis of blood formation positive regulators, which were determined in the bone marrow cells supernatant after radiation exposure, the PETE usage promoted successful and more rapid recovery of hematopoiesis, creating in turn the preconditions for effective immunogenesis.