Determination of partition coefficients of antiglaucoma drugs between erythrocytes and the medium
Keywords:
erythrocyte, shape, antiglaucoma drugs, partition coefficien, bilayer couple theory
Abstract
In this work, the effect of some antiglaucoma drugs on the shape of human erythrocytes was investigated. It
has been shown that increasing drug concentration leads to shape transformation of erythrocytes from
discs toward spheres. Using the established dependence of shape index versus drug concentration in the
medium the method to measure a free drug concentration based on the implementation of erythrocytes as
a biological sensor was developed. This assay was further employed to determine partition coefficients for
erythrocytes and pink ghosts which did not vary significantly among different drugs and had an order of
1⋅103. For Benzalkonium chloride, which is a common agent for all drugs an estimate of the number of
molecules bound to the red blood cell membrane and an increase in its surface area depending on the shape of the cell has been done.
Downloads
Download data is not yet available.
References
1. Hoffman J.F. On the mechanism and measurement of shape transformations of constant volume of human red blood cells, Blood Cells. 1987, 12, 565-588.
2. Hoffman J.F. Some red blood cell phenomena for the curious, Blood Cells Mol.Dis. 2004, 32, 335-340.
3. Wong P. A basis of echinocytosis and stomatocytosis in the disc-sphere transformations of the erythrocyte, J.Theor.Biol. 1999, 196, 343-61.
4. Wong P A hypothesis of the disc-sphere transformation of the erythrocytes between glass surfaces and of related observations, J.Theor.Biol. 2005, 233, 127-135.
5. Hoffman J.F. Quantitative study of factors which control shape transformations of human red blood cells of constant volume, Nouv.Rev.Fr.Hematol. 1972, 12, 771-774.
6. Sheetz M.P., Singer S.J. Biological membranes as bilayer couples. A molecular mechanism of drug- erythrocyte interactions, Proc.Natl.Acad.Sci.U.S.A 1974, 71, 4457-4461.
7. Lim H.W.G., Wortis M., Mukhopadhyay R. Stomatocyte-discocyte-echinocyte sequence of the human red blood cell: evidence for the bilayer- couple hypothesis from membrane mechanics, Proc.Natl.Acad.Sci.U.S.A 2002, 99, 16766-16769.
8. Mukhopadhyay R, Lim H.W.G., Wortis M. , Echinocyte shapes: bending, stretching, and shear determine spicule shape and spacing, Biophys.J. 2002, 82, 1756-1772.
9. Chi L.M., Wu W.G., Effective bilayer expansion and erythrocyte shape change induced by monopalmitoyl phosphatidylcholine. Quantitative light microscopy and nuclear magnetic resonance spectroscopy measurements, Biophys.J. 1990, 57, 1225-1232.
10. Ferrell J.E.J., Lee K.J, Huestis W.H. Membrane bilayer balance and erythrocyte shape: a quantitative assessment, Biochemistry 1985, 24, 2849-2857.
11. Sheetz M.P., Painter R.G., Singer S.J., Biological membranes as bilayer couples. III. Compensatory shape changes induced in membranes, J.Cell Biol. 1976, 70, 193-203.
12. Sheetz M.P., Singer S.J. Equilibrium and kinetic effects of drugs on the shapes of human erythrocytes, J.Cell Biol. 1976, 70, 247-251.
13. Iglic A, Kralj-Iglic V, Hagerstrand H, Amphiphile induced echinocyte-spheroechinocyte transformation of red blood cell shape, Eur.Biophys.J. 1998, 27, 335-339.
14. Lange Y, Slayton J.M. Interaction of cholesterol and lysophosphatidylcholine in determining red cell shape, J.Lipid Res. 1982, 23, 1121-1127.
15. Sheetz M.P., Singer S.J. Biological membranes as bilayer couples. A molecular mechanism of drug- erythrocyte interactions, Proc.Natl.Acad.Sci.U.S.A 1974,.71, 4457-61.
16. Руденко С.В., Мухамед Хани Румиех, Бондаренко В.А. Морфологическая реакция эритроцитов на изменение электролитного состава среды. II. Влияние ингибиторов анионного транспорта, Вісник Харківського національного університету імені В.Н. Каразіна. Біофізічний вісник. 2007, Вип. 1(18),. 53- 60.
17. Rudenko S.V., Nipot E.E. Protection by chlorpromazine, albumin and divalent cations of hemolysis induced by melittin, [ala-14]melittin and whole bee venom, Biochem. J. 1996, 317, 747-754.
18. Rudenko S.V., Crowe J.H., Tablin F. Determination of time-dependent shape changes in red blood cells, Biochemistry (Mosc.) 1998, 63, 1385-1394.
19. Ивков В.Г., Берестовский Г.Н. Динамическая структура липидного бислоя, М.: Наука, 1981, 296 с.
20. Hartmann J, Glaser R. The influence of chlorpromazine on the potential-induced shape change of human erythrocyte, Biosci.Rep. 1991, 11, 213-221.
2. Hoffman J.F. Some red blood cell phenomena for the curious, Blood Cells Mol.Dis. 2004, 32, 335-340.
3. Wong P. A basis of echinocytosis and stomatocytosis in the disc-sphere transformations of the erythrocyte, J.Theor.Biol. 1999, 196, 343-61.
4. Wong P A hypothesis of the disc-sphere transformation of the erythrocytes between glass surfaces and of related observations, J.Theor.Biol. 2005, 233, 127-135.
5. Hoffman J.F. Quantitative study of factors which control shape transformations of human red blood cells of constant volume, Nouv.Rev.Fr.Hematol. 1972, 12, 771-774.
6. Sheetz M.P., Singer S.J. Biological membranes as bilayer couples. A molecular mechanism of drug- erythrocyte interactions, Proc.Natl.Acad.Sci.U.S.A 1974, 71, 4457-4461.
7. Lim H.W.G., Wortis M., Mukhopadhyay R. Stomatocyte-discocyte-echinocyte sequence of the human red blood cell: evidence for the bilayer- couple hypothesis from membrane mechanics, Proc.Natl.Acad.Sci.U.S.A 2002, 99, 16766-16769.
8. Mukhopadhyay R, Lim H.W.G., Wortis M. , Echinocyte shapes: bending, stretching, and shear determine spicule shape and spacing, Biophys.J. 2002, 82, 1756-1772.
9. Chi L.M., Wu W.G., Effective bilayer expansion and erythrocyte shape change induced by monopalmitoyl phosphatidylcholine. Quantitative light microscopy and nuclear magnetic resonance spectroscopy measurements, Biophys.J. 1990, 57, 1225-1232.
10. Ferrell J.E.J., Lee K.J, Huestis W.H. Membrane bilayer balance and erythrocyte shape: a quantitative assessment, Biochemistry 1985, 24, 2849-2857.
11. Sheetz M.P., Painter R.G., Singer S.J., Biological membranes as bilayer couples. III. Compensatory shape changes induced in membranes, J.Cell Biol. 1976, 70, 193-203.
12. Sheetz M.P., Singer S.J. Equilibrium and kinetic effects of drugs on the shapes of human erythrocytes, J.Cell Biol. 1976, 70, 247-251.
13. Iglic A, Kralj-Iglic V, Hagerstrand H, Amphiphile induced echinocyte-spheroechinocyte transformation of red blood cell shape, Eur.Biophys.J. 1998, 27, 335-339.
14. Lange Y, Slayton J.M. Interaction of cholesterol and lysophosphatidylcholine in determining red cell shape, J.Lipid Res. 1982, 23, 1121-1127.
15. Sheetz M.P., Singer S.J. Biological membranes as bilayer couples. A molecular mechanism of drug- erythrocyte interactions, Proc.Natl.Acad.Sci.U.S.A 1974,.71, 4457-61.
16. Руденко С.В., Мухамед Хани Румиех, Бондаренко В.А. Морфологическая реакция эритроцитов на изменение электролитного состава среды. II. Влияние ингибиторов анионного транспорта, Вісник Харківського національного університету імені В.Н. Каразіна. Біофізічний вісник. 2007, Вип. 1(18),. 53- 60.
17. Rudenko S.V., Nipot E.E. Protection by chlorpromazine, albumin and divalent cations of hemolysis induced by melittin, [ala-14]melittin and whole bee venom, Biochem. J. 1996, 317, 747-754.
18. Rudenko S.V., Crowe J.H., Tablin F. Determination of time-dependent shape changes in red blood cells, Biochemistry (Mosc.) 1998, 63, 1385-1394.
19. Ивков В.Г., Берестовский Г.Н. Динамическая структура липидного бислоя, М.: Наука, 1981, 296 с.
20. Hartmann J, Glaser R. The influence of chlorpromazine on the potential-induced shape change of human erythrocyte, Biosci.Rep. 1991, 11, 213-221.
Published
2009-06-05
Cited
How to Cite
Rudenko, S. V., & Saeid, M. K. (2009). Determination of partition coefficients of antiglaucoma drugs between erythrocytes and the medium. Biophysical Bulletin, 2(23), 69-76. Retrieved from https://periodicals.karazin.ua/biophysvisnyk/article/view/4366
Section
Cell biophysics
Copyright (c) 2009 S.V. Rudenko, Mohammed H. Said
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
